① Upload
② Plates
③ Layout
④ Settings
⑤ Preview
⑥ Export

Upload ELISA Data

CSV from any 96-well plate reader — single or multi-plate, any wavelengths.

🧫

Drop CSV here or click to browse

Automatically detects wavelengths and plate boundaries

Session

Save or restore a full session (layouts, groups, settings).

Sample Input File

Download a generic example of the raw plate reader CSV format this converter expects.

Configure Plates

Set plate metadata. Immunogen can be overridden per sample group in the Layout step.

Experimental Variables

Define variables that differ across wells on this plate (e.g. Secondary Antibody, Antigen). Wells will be tagged with these variables in the Layout step. Each unique combination becomes a separate experiment in the export.

Well Assignment

Drag to select wells. Assign type, group, dilution and variables using the panels.

Select:
Assign:
Blank
PBS ctrl
Pos ctrl
Neg ctrl
Unused

Assign Wells

Select wells on the grid to assign them.
Well Type
Or assign as Sample
Dilution Assignment
Series
Manual
None

Groups

No groups yet.

Assign Variables

No variables defined. Add in Step 1.

Partitions

Each variable combination = one experiment. Click colour to change.

Inspector

Hover over a well to inspect.

Layout Templates

Processing Settings

Configure wavelength selection, background subtraction, and output naming.

Wavelength

Subtract reference wavelength

Background Subtraction

Subtract blank well mean from all values

Experiment Name Template

Uses {immunogen} (from group name) and {antigen} (from plate metadata).

Preview:

Replicate Handling

Data Preview

Verify processed results. Negative values shown in red. Missing replicates highlighted.

Processed Table

Export

Download structured CSV. All plates combined.

Options

Preview

Output Filename

.csv